Police said there were no casualties following the blast at Mount Lavinia, but the Colombo-bound track was damaged and train services were suspended pending investigation.
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"Fortunately there was no train passing at the time," a police official at the scene said. "A part of the track was ripped off and we need to check for any more damage to the track.
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There was no immediate claim of responsibility, but police said they were investigating the involvement of Tamil Tiger rebels who are facing a major military offensive against them in the north of the island.
The Tigers have been blamed for a spate of similar attacks in the past.